Created
September 24, 2015 18:48
-
-
Save bmiles/a9a4ecf9f32448e2a348 to your computer and use it in GitHub Desktop.
This file is an example of a simple bacterial incubation and OD600 experiment documented in the Autoprotocol JSON data standard.
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
| { | |
| "refs": { | |
| "growth_plate": { | |
| "id": "3208kfhf394", | |
| "store": { | |
| "where": "cold_4" | |
| } | |
| }, | |
| "DH5a": { | |
| "discard": true, | |
| "id": "3209xifd029" | |
| } | |
| }, | |
| "instructions": [ | |
| { | |
| "reagent": "lb-broth-noAB", | |
| "object": "growth_plate", | |
| "columns": [ | |
| { | |
| "column": "0", | |
| "volume": "150:microliter" | |
| } | |
| ], | |
| "op": "dispense" | |
| }, | |
| { | |
| "groups": [ | |
| { | |
| "transfer": [ | |
| { | |
| "volume": "2.0:microliter", | |
| "to": "growth_plate/0", | |
| "from": "DH5a/0" | |
| } | |
| ] | |
| }, | |
| { | |
| "transfer": [ | |
| { | |
| "volume": "2.0:microliter", | |
| "to": "growth_plate/12", | |
| "from": "DH5a/0" | |
| } | |
| ] | |
| }, | |
| { | |
| "transfer": [ | |
| { | |
| "volume": "2.0:microliter", | |
| "to": "growth_plate/24", | |
| "from": "DH5a/0" | |
| } | |
| ] | |
| }, | |
| { | |
| "transfer": [ | |
| { | |
| "volume": "2.0:microliter", | |
| "to": "growth_plate/36", | |
| "from": "DH5a/0" | |
| } | |
| ] | |
| } | |
| ], | |
| "op": "pipette" | |
| }, | |
| { | |
| "lid": "low_evaporation", | |
| "object": "growth_plate", | |
| "op": "cover" | |
| }, | |
| { | |
| "where": "warm_37", | |
| "object": "growth_plate", | |
| "co2_percent": 0, | |
| "duration": "12:hour", | |
| "shaking": true, | |
| "op": "incubate" | |
| }, | |
| { | |
| "dataref": "OD600", | |
| "object": "growth_plate", | |
| "wells": ["A1", "B1", "C1", "D1", "E1", "F1", "G1", "H1"], | |
| "num_flashes": 25, | |
| "wavelength": "600:nanometer", | |
| "op": "absorbance" | |
| } | |
| ] | |
| } |
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
Generated from this code https://gist.github.com/bmiles/7c15436666e89cb6c194