This document examines how to use RELION 4.0 (beta2 as of writing) with Warp 1.09 and M 1.09 for single particle analysis.
Special thanks to Alister Burt, Pranav Shah and Dimitry Tegunov for discussion on this Twitter thread.
We use the RELION tutorial dataset (beta-galactosidase collected on JEOL CRYO ARM 200, a subset of EMPIAR-10204).