Authors: Ayushi Kamthan, Mohan Kamthan, Niranjan Chakraborty, Subhra Chakraborty & Asis Datta
Gas chromatography- mass spectrometry (GC-MS) is a highly used and well established technological platform for metabolite profiling in both plant and non-plant species. Here we describe a simple protocol for lipophilic compounds extraction and analysis from tomato fruits and leaves which is an important crop in terms of its economic and nutritional value. This is a rapid, reliable and reproducible method to detect and quantify lipophilic metabolites including fatty acids, fatty alcohols, sterols, alkanes, alkenes, and triterpenoids by GC-MS. In addition, this is a simple and convenient method for efficient comparative lipid profiling of wild type and transgenic or mutant tomato fruits.
Metabolomics is one of the most rapidly advancing analytical approaches that aim at the comprehensive analysis of a large numbers of metabolites. It is most extensively utilized method in the fiel