Mac Cowell 100ideas

#OpenTrons Protocol Datastructure ALPHA ###(SUBJECT TO CHANGE)

The OT.One runs jobs based on a JSON datastructure. This "low-level" definiton provides volume, container, and location specific information, describing protocols at the liquid transfer level (as opposed to Barista's higher level description).

It is based loosely on [Transcriptic's Autoprotocol] (https://www.transcriptic.com/platform/#instructions). We modified it to suit platforms beyond Transcriptic's robotic work cells, parsing out the platform specific information into a separate file with information particular to the OT platform. This should empower biodevelopers to easily develop and test protocols on their local machine, and then quickly scale them to "the cloud."

Please send any questions to info@opentrons.com!

Screencapture and animated gifs

I say "animated gif" but in reality I think it's irresponsible to be serving "real" GIF files to people now. You should be serving gfy's, gifv's, webm, mp4s, whatever. They're a fraction of the filesize making it easier for you to deliver high fidelity, full color animation very quickly, especially on bad mobile connections. (But I suppose if you're just doing this for small audiences (like bug reporting), then LICEcap is a good solution).

Capturing (Easy)

Launch quicktime player
do Screen recording

How to connect PS3 controller to a Mac or PC

This is how you connect PS3 controller to Mac OSX, PC, etc. when previously connected to a PS3. You will need a Mini USB cable. Overcome your laziness, get up of your chair, and go get one!

A big misconception is that keep holding PS button will reset the controller's pairing. It DOES NOT! From my testings, the controller keeps paring with the last machine it was CONNECTED VIA A USB CABLE.

Here are the steps:

	#!/usr/bin/env bash
	#
	#!/usr/bin/env bash
	#: Usage: brew lsbrews [OPTION]
	#:
	#: list name: description of all instealled "leaf" formulae
	#:
	# disables logging the script's contents to .bash_history
	set +o history

	"""
	Convert YouTube subtitles(vtt) to human readable text.

	Download only subtitles from YouTube with youtube-dl:
	youtube-dl --skip-download --convert-subs vtt <video_url>

	Note that default subtitle format provided by YouTube is ass, which is hard
	to process with simple regex. Luckily youtube-dl can convert ass to vtt, which
	is easier to process.

	from autoprotocol.protocol import Protocol

	p = Protocol()
	# Set up the containers being referenced
	ctrl_bacteria = p.ref("DH5a", id="3209xifd029", cont_type="micro-1.5", discard=True)
	growth_plate = p.ref("growth_plate", id="3208kfhf394", cont_type="96-flat", storage="cold_4")

	# Dispense, transfer, cover the plate, incubate, measure OD600
	p.dispense(growth_plate, "lb-broth-noAB", [{"column": "0", "volume": "150:microliter"}])
	p.transfer(ctrl_bacteria.well(0), growth_plate.wells_from(0,4, columnwise=True), "2:microliter")

	import requests, re, json, types, traceback

	class HypothesisAnnotation:

	def __init__(self, row):
	"""Encapsulate relevant parts of one row of a Hypothesis API search."""

	self.tags = []
	if row.has_key('tags') and row['tags'] is not None:
	self.tags = row['tags']

	package main

	import (
	"encoding/json"
	"fmt"
	"log"
	"net/http"
	"net/url"
	"os/exec"
	"strconv"

	<html>
	<head>
	<script src="http://d3js.org/d3.v3.min.js"></script>
	</head>
	<body>
	<style>

	#chart svg {
	height: 400px;
	}